Abstract
This study compares two harmful red tide (Karenia brevis) events along Florida’s west coast—October 2022–February 2023 (no major hurricane) and October 2024–February 2025 following Hurricane Milton—using Sentinel-2 multispectral imagery processed in Google Earth Engine and ENVI. Monthly median composites (October, December, February) over an ~835 km² region near Sanibel Island were analyzed with four spectral indices (NDCI, Red Tide Index, Chlorophyll Index, and Floating Algae Index), followed by a supervised Maximum Likelihood classification to map three classes: red algae, water without algae, and land. The Floating Algae Index provided the clearest separation, while NDCI was confounded by terrestrial vegetation. Contrary to the initial hypothesis that hurricane-driven nutrient pulses would amplify blooms, the post-hurricane period showed consistently smaller algae extent than the 2022–2023 case (reductions of ~2,800 ha in October, ~20,600 ha in December, and ~14,800 ha in February). Potential explanations include classification and mosaic artifacts, flood-related land/water confusion, and nutrient flushing. The study demonstrates the utility of Sentinel-2 for HAB monitoring and recommends broader spatial/temporal coverage and integration with in-situ measurements for robust attribution.